Dosage Form of Moisturizer Gel and Evaluation of Antibacterial, Anti-inflammatory, Antioxidant of Erythrina crista-galli L. Flowers Extract
Abstract
Objective: This study aimed to determine the antioxidant, antibacterial, and anti-inflammatory activities of ethanol extracts from red Dadap flowers (Erythrina crista-galli L). In addition to formulate the extract into a moisturizing gel preparation of good physical quality.
Material and Methods: Antioxidant activity was determined using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity assay. Antibacterial activity was evaluated using the disc diffusion method. Anti-inflammatory activity was evaluated using carrageenan-induced paw edema in mice in vivo. Additionally, in silico studies were performed and the physical quality of the extract-gel formulation was evaluated.
Results: Antioxidant testing resulted in a half-maximal inhibitory concentration (IC50) value of 79.1 ppm and an AAI value of 0.4. Antibacterial testing obtained the largest inhibition zone, with an average of 9.0 mm. Anti-inflammatory testing obtained the greatest anti-inflammatory effect at a dose of 300 mg/kg body weight in mice, with a percentage of inflammation inhibition that reached 59.32%. In silico testing, the smallest binding affinity values were obtained: respectively, at -10.3, -12.0, and -10.2 kcal/mol, by the specific compound Cyanidin-3-O-glucoside. Physical quality evaluation obtained values that are in accordance with the Indonesian National Standard.
Conclusion: Ethanol extract of red Dadap flower (Erythrina crista-galli L.) has antioxidant and antibacterial activity in the weak category; however, in contrast it has anti-inflammatory activity in the strong category. In silico also obtained good activity in accordance with the results of in vivo testing. Erythrina crista-galli L. flower extract can be formulated into a moisturizer gel, this can be seen from the results of good physical stability.
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